Effect of high cholesterol diet on tissue levels of riboflavin and choline.

In our recent investigation we have studied the effect of high dose of choline and riboflavin on the fecal excretion of cholesterol and bile acids of albino rats kept on a high cholesterol diet and found that choline increased the fecal excretion of bile acid without showing any appreciable in crease in the excretion of cholesterol in feces. Riboflavin did not show any appreci able increase or decrease in the excretion of cholesterol and bile acid in the feces of the animals fed a high cholesterol diet. Both choline and riboflavin exerted a cholesterol lowering effect in the liver and blood of the animals fed high cholesterol diet (1). Reiser and Pearson (2) have observed an increased requirement of riboflavin in chicks fed cotton seed oil. Borglin (3) reported an increase in choline requirement in high fat fed human beings. As high fat diet influences the re quirement of riboflavin and choline respec tively, we thought it worth-while to investigate whether high cholesterol diet influences the requirement of riboflavin and choline of albino rats. EXPERIMENTAL